Dataset Information

Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway.

ABSTRACT: It has been reported that double-stranded break (DSB)-induced small RNAs (diRNAs) are generated via the RNA-directed DNA methylation pathway and function in DSB repair in Arabidposis. However, important questions remain regarding the biogenesis and function of diRNAs. Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to characterize diRNAs in Arabidopsis and rice. We found that 21-nt diRNAs were generated from a 35S promoter::GU-US reporter transgene targeted by CRISPR/Cas9. Unexpectedly, Pol II transcription of the transgene was required for efficient diRNA production and the level of diRNA accumulation correlated with the expression level of the transgene. diRNAs were not detected from CRISPR/Cas9- or TALEN-induced DSBs within the examined endogenous genes in Arabidopsis or rice. We also found that DCL4 and RDR6 that are known to be involved in posttranscriptional gene silencing were required to generate diRNAs. Our results suggest that DSBs are necessary but not sufficient for efficient diRNA generation and a high level of diRNAs is not necessary for DSB repair.

SUBMITTER: Miki D

PROVIDER: S-EPMC5428250 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway.

Miki Daisuke D Zhu Peiying P Zhang Wencan W Mao Yanfei Y Feng Zhengyan Z Huang Huan H Zhang Hui H Li Yanqiang Y Liu Renyi R Zhang Huiming H Qi Yijun Y Zhu Jian-Kang JK

Scientific reports 20170322 1

It has been reported that double-stranded break (DSB)-induced small RNAs (diRNAs) are generated via the RNA-directed DNA methylation pathway and function in DSB repair in Arabidposis. However, important questions remain regarding the biogenesis and function of diRNAs. Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to characterize diRNAs in Arabidopsis and rice. We found that 21-nt diRNAs were generated from a 35S promoter::GU-US reporter transgene targeted by CRISPR/Cas9. Unexpectedly, Pol I ...[more]

PMID: 28331197

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Dataset Information

Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway.

Publications

Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway.

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