Dataset Information

Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach.

ABSTRACT: Cold environments dominate Earth's biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein, we assessed the metabolic features of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125), a model organism for cold-adaptation, at both 4?°C and 15?°C, by integrating genomic and phenomic (high-throughput phenotyping) data and comparing the obtained results to the taxonomically related Antarctic bacterium Pseudoalteromonas sp. TB41 (PspTB41). Although the genome size of PspTB41 is considerably larger than PhTAC125, the higher number of genes did not reflect any higher metabolic versatility at 4?°C as compared to PhTAC125. Remarkably, protein S-thiolation regulated by glutathione and glutathionylspermidine appeared to be a new possible mechanism for cold adaptation in PhTAC125. More in general, this study represents an example of how 'multi-omic' information might potentially contribute in filling the gap between genotypic and phenotypic features related to cold-adaptation mechanisms in bacteria.

SUBMITTER: Mocali S

PROVIDER: S-EPMC5429795 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach.

Mocali Stefano S Chiellini Carolina C Fabiani Arturo A Decuzzi Silvia S de Pascale Donatella D Parrilli Ermenegilda E Tutino Maria Luisa ML Perrin Elena E Bosi Emanuele E Fondi Marco M Lo Giudice Angelina A Fani Renato R

Scientific reports 20170412 1

Cold environments dominate Earth's biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein, we assessed the metabolic features of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125), a model organism for cold-adaptation, at both 4 °C and 15 °C, by integrating genomic and phenom ...[more]

PMID: 28404986

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Dataset Information

Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach.

Publications

Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach.

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