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Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation.

ABSTRACT: We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method, demonstrated by the renaturation of several enzymes, is efficient, rapid, employs a minimal amount of reagents and even can be applied to renature mixture of the denatured enzymes.

SUBMITTER: Volodina KV

PROVIDER: S-EPMC5431136 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation.

Volodina Katerina V KV Avnir David D Vinogradov Vladimir V VV

Scientific reports 20170503 1

We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method, demonstrated by the renaturation of several enzymes, is efficient, rapid, employs a minimal amount of reagents and even can be applied to renature mixture of the denatured enzymes. ...[more]

PMID: 28469182

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