Ontology highlight
ABSTRACT:
SUBMITTER: Lei C
PROVIDER: S-EPMC5436000 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Lei Chao C Li Shi-Yuan SY Liu Jia-Kun JK Zheng Xuan X Zhao Guo-Ping GP Wang Jin J
Nucleic acids research 20170501 9
As Cpf1 cleaves double-stranded DNA in a staggered way, it can be used in DNA assembly. However, the Cpf1 cleavage was found to be inaccurate, which may cause errors in DNA assembly. Here, the Cpf1 cleavage sites were precisely characterized, where the cleavage site on the target strand was around the 22nd base relative to the protospacer adjacent motif site, but the cleavage on the non-target strand was affected by the spacer length. When the spacer length was 20 nt or longer, Cpf1 mainly cleav ...[more]