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PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.

ABSTRACT: PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase ? with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling.

SUBMITTER: Daigaku Y

PROVIDER: S-EPMC5440044 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.

Daigaku Yasukazu Y Etheridge Thomas J TJ Nakazawa Yuka Y Nakayama Mayumi M Watson Adam T AT Miyabe Izumi I Ogi Tomoo T Osborne Mark A MA Carr Antony M AM

PLoS genetics 20170508 5

PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ...[more]

PMID: 28481910

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