Identification of function-regulating antibodies targeting the receptor protein tyrosine phosphatase sigma ectodomain.

ABSTRACT: Receptor tyrosine phosphatase sigma (RPTP?) plays an important role in the regulation of axonal outgrowth and neural regeneration. Recent studies have identified two RPTP? ligands, chondroitin sulfate proteoglycans (CSPGs) and heparan sulfate proteoglycans (HSPG), which can modulate RPTP? activity by affecting its dimerization status. Here, we developed a split luciferase assay to monitor RPTP? dimerization in living cells. Using this system, we demonstrate that heparin, an analog of heparan sulfate, induced the dimerization of RPTP?, whereas chondroitin sulfate increased RPTP? activity by inhibiting RPTP? dimerization. Also, we generated several novel RPTP? IgG monoclonal antibodies, to identify one that modulates its activity by inducing/stabilizing dimerization in living cells. Lastly, we demonstrate that this antibody promotes neurite outgrowth in SH-SY5Y cells. In summary, we demonstrated that the split luciferase RPTP? activity assay is a novel high-throughput approach for discovering novel RPTP? modulators that can promote axonal outgrowth and neural regeneration.

SUBMITTER: Wu CL 

PROVIDER: S-EPMC5449173 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature