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A reverse genetic screen in Drosophila using a deletion-inducing mutagen.

ABSTRACT: We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.

SUBMITTER: Nairz K

PROVIDER: S-EPMC545603 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature

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A reverse genetic screen in Drosophila using a deletion-inducing mutagen.

Nairz Knud K Zipperlen Peder P Dearolf Charles C Basler Konrad K Hafen Ernst E

Genome biology 20040928 10

We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to oth ...[more]

PMID: 15461801

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