A genome annotation-driven approach to cloning the human ORFeome.
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ABSTRACT:
SUBMITTER: Collins JE
PROVIDER: S-EPMC545604 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Publications
A genome annotation-driven approach to cloning the human ORFeome.
Collins John E JE Wright Charmain L CL Edwards Carol A CA Davis Matthew P MP Grinham James A JA Cole Charlotte G CG Goward Melanie E ME Aguado Begoña B Mallya Meera M Mokrab Younes Y Huckle Elizabeth J EJ Beare David M DM Dunham Ian I
Genome biology 20040930 10
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined. ...[more]
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