Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Municipal wastewater treatment by the bioaugmentation of Bacillus sp. K5 within a sequencing batch reactor.

ABSTRACT: Artificial municipal wastewater was treated successfully by the bioaugmentation of Bacillus sp. K5 capable of simultaneous nitrification and denitrification (SND) within a sequencing batch reactor (SBR). During the long-term operation, the bioaugmentation system exhibited an excellent and steady COD and NH4+-N removal without nitrite and nitrate accumulation. The average removal efficiency for COD and NH4+-N achieved to 98% and 95%, respectively. PCR-DGGE, SEM and FISH revealed that the introduced Bacillus sp. K5 should be an important functional strain and exerted a critical influence on the structure of microbial community. qPCR analysis indicated that the strain K5 facilitated aerobic nutrients removal capabilities and SND might be the primary pathway for the nitrogen removal in the SBR. Overall, the SBR system used in our study should be very promising for the future wastewater treatment.

SUBMITTER: Yang Y

PROVIDER: S-EPMC5464570 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Municipal wastewater treatment by the bioaugmentation of Bacillus sp. K5 within a sequencing batch reactor.

Yang Yunlong Y Xie Linxiang L Tao Xin X Hu Kaihui K Huang Shaobin S

PloS one 20170608 6

Artificial municipal wastewater was treated successfully by the bioaugmentation of Bacillus sp. K5 capable of simultaneous nitrification and denitrification (SND) within a sequencing batch reactor (SBR). During the long-term operation, the bioaugmentation system exhibited an excellent and steady COD and NH4+-N removal without nitrite and nitrate accumulation. The average removal efficiency for COD and NH4+-N achieved to 98% and 95%, respectively. PCR-DGGE, SEM and FISH revealed that the introduc ...[more]

PMID: 28594856

Similar Datasets

Modelling and parameter optimisation for performance evaluation of sequencing batch reactor for treating hospital wastewater.

Project description:Hospital wastewater treatment is gaining attention in recent studies due to its complex nature. The performance of the sequencing batch reactor coupled with tube-settler was investigated for hospital wastewater treatment. The performance was evaluated regarding removing organic matter and nutrients (nitrate and phosphate). The phosphate was removed in the sequencing batch reactor and its associated tube-settler with a 60% removal efficiency margin. Nitrification was observed in sequencing batch reactor and tube-settler, but denitrification could not be achieved. The nitrification-denitrification process was not completed during the process. The current work's main aim was to understand and optimise the operational parameters involved in the performance of the sequencing batch reactor. The operational parameters were optimised using Design expert software, and Response Surface Methodology involved a four-factor and five-level central composite design. The percentage removal of chemical oxygen demand, nitrate, and phosphate was selected to be observed during this study.Graphical abstractSupplementary informationThe online version contains supplementary material available at 10.1007/s13399-022-03406-z.

| S-EPMC9628615 | biostudies-literature

Microbial community and performance of a partial nitritation/anammox sequencing batch reactor treating textile wastewater.

Project description:Implementation of onsite bioremediation technologies is essential for textile industries due to rising concerns in terms of water resources and quality. Partial nitritation-anaerobic ammonium oxidation (PN/A) processes emerged as a valid, but unexplored, solution. In this study, the performance of a PN/A pilot-scale (9 m3) sequencing batch reactor treating digital textile printing wastewater (10-40 m3 d-1) was monitored by computing nitrogen (N) removal rate and efficiencies. Moreover, the structure of the bacterial community was assessed by next generation sequencing and quantitative polymerase chain reaction (qPCR) analyses of several genes, which are involved in the N cycle. Although anaerobic ammonium oxidation activity was inhibited and denitrification occurred, N removal rate increased from 16 to 61 mg N g VSS-1 d-1 reaching satisfactory removal efficiency (up to 70%). Ammonium (18-70 mg L-1) and nitrite (16-82 mg L-1) were detected in the effluent demonstrating an unbalance between the aerobic and anaerobic ammonia oxidation activity, while constant organic N was attributed to recalcitrant azo dyes. Ratio between nitrification and anammox genes remained stable reflecting a constant ammonia oxidation activity. A prevalence of ammonium oxidizing bacteria and denitrifiers suggested the presence of alternative pathways. PN/A resulted a promising cost-effective alternative for textile wastewater N treatment as shown by the technical-economic assessment. However, operational conditions and design need further tailoring to promote the activity of the anammox bacteria.

| S-EPMC8637490 | biostudies-literature

Partial Nitrification and Enhanced Biological Phosphorus Removal in a Sequencing Batch Reactor Treating High-Strength Wastewater.

Project description:Complex and high levels of various pollutants in high-strength wastewaters hinder efficient and stable biological nutrient removal. In this study, the changes in pollutant removal performance and microbial community structure in a laboratory-scale anaerobic/aerobic sequencing batch reactor (SBR) treating simulated pre-fermented high-strength wastewater were investigated under different influent loading conditions. The results showed that when the influent chemical oxygen demand (COD), total nitrogen (TN), and orthophosphate (PO43--P) concentrations in the SBR increased to 983, 56, and 20 mg/L, respectively, the COD removal efficiency was maintained above 85%, the TN removal efficiency was 64.5%, and the PO43--P removal efficiency increased from 78.3% to 97.5%. Partial nitrification with simultaneous accumulation of ammonia (NH4+-N) and nitrite (NO2--N) was observed, which may be related to the effect of high influent load on ammonia- and nitrite-oxidising bacteria. The biological phosphorus removal activity was higher when propionate was used as the carbon source instead of acetate. The relative abundance of glycogen accumulating organisms (GAOs) increased significantly with the increase in organic load, while Tetrasphaera was the consistently dominant polyphosphate accumulating organism (PAO) in the reactor. Under high organic loading conditions, there was no significant PAO-GAO competition in the reactor, thus the phosphorus removal performance was not affected.

| S-EPMC9105176 | biostudies-literature

Efficiency of sequencing batch reactor for removal of organic matter in the effluent of petroleum wastewater.

Project description:The main aim of this research was to study the biodegradation of Methyl Tertiary Butyl Ether (MTBE) using aerobic sequencing batch reactor (SBR) at a pilot-Scale. The reactor was made of a 3 mm-thick glass cylinder with an internal diameter of 12 cm and height of 60 cm. SBR operated in five phases. The first phase was filling the reactor for about 10 min. the second phase was the main reactor for biological treatment of petroleum wastewater about 21.55 h. The third phase was the sedimentation (1 h). The fourth phase was decanting from the reactor for about 10 min. The last phase consisted of idle for about 45 min. The experiments showed that the mixed microbial mass is able to degrade high concentration of methanol up to 250 mg/l, and concentration of MTBE up to 70 mg/l for a 24 h cycle. However, the mixed microbial mass is not able to degrade MTBE with concentration more than 70 mg/l. Microorganisms were generally isolated from Fajr petrochemical wastewater treatment plant. Analysis showed that the mixed microbial mass able to biodegradation of COD up to 1350 mg/l in effluent. Aerobic SBR can be used for biological treatment of the petroleum wastewater containing pollutants such as methanol, MTBE with a promising efficiency.

| S-EPMC6141387 | biostudies-literature

The effects of salinity on nitrification using halophilic nitrifiers in a Sequencing Batch Reactor treating hypersaline wastewater.

Project description:With annual increases in the generation and use of saline wastewater, the need to avoid environmental problems such as eutrophication is critical. A previous study identified ways to start up a halophilic sludge domesticated from estuarine sediments to remove nitrogen from wastewater with a salinity of 30?g/L. This investigation expands that work to explore the impact of salinity on nitrogen removal. This study demonstrated that the mixed halophilic consortia removed nitrogen from wastewater with a salinity of 30-85?g/L. A kinetic analysis showed that halophilic nitrifiers selected based on hypersalinity were characterized by low Ks, ?max and specific ammonium oxidization rates. This explains the decrease in ammonium removal efficiency in the high salinity operational phases. Salinity inhibited ammonia oxidizing bacteria (AOB) activity, as well as the number of dominant AOB, but did not significantly affect the AOB dominant species. Three most dominant AOB lineages in the halophilic sludge were Nitrosomonas marina, Nitrosomonas europaea, and Nitrosococcus mobilis. Nitrosomonas europaea and Nitrosococcus mobilis were mainly affected by salinity, while nitrite accumulation and ammonia loading played the key role in determining the abundance of Nitrosococcus mobilis and Nitrosococcus europaea. The study contributes insights about shifts in halophilic nitrifying bacterial populations.

| S-EPMC4843016 | biostudies-literature

Nitrifying granules cultivation in a sequencing batch reactor at a low organics-to-total nitrogen ratio in wastewater.

Project description:It is possible to cultivate aerobic granular sludge at a low organic loading rate and organics-to-total nitrogen (COD/N) ratio in wastewater in the reactor with typical geometry (height/diameter = 2.1, superficial air velocity = 6 mm/s). The noted nitrification efficiency was very high (99%). At the highest applied ammonia load (0.3 ± 0.002 mg NH (4) (+) -N g total suspended solids (TSS)(-1) day(-1), COD/N = 1), the dominating oxidized form of nitrogen was nitrite. Despite a constant aeration in the reactor, denitrification occurred in the structure of granules. Applied molecular techniques allowed the changes in the ammonia-oxidizing bacteria (AOB) community in granular sludge to be tracked. The major factor influencing AOB number and species composition was ammonia load. At the ammonia load of 0.3 ± 0.002 mg NH (4) (+) -N g TSS(-1) day(-1), a highly diverse AOB community covering bacteria belonging to both the Nitrosospira and Nitrosomonas genera accounted for ca. 40% of the total bacteria in the biomass.

| S-EPMC3131513 | biostudies-literature

Pilot plant study on nitrogen and phosphorus removal in marine wastewater by marine sediment with sequencing batch reactor.

Project description:Effective biological treatment of marine wastewater is not well-known. Accumulation of nitrogen and phosphorus from land-based effluent is a crucial cause of red-tide in marine systems. The purpose of the study is to reduce nitrogen and phosphorus in marine wastewater with a pilot plant-scale sequencing batch reactor (SBR) system by using marine sediment as eco-friendly and effective biological materials, and elucidate which bacterial strains in sludge from marine sediment influence the performance of SBR. By applying eco-friendly high efficiency marine sludge (eco-HEMS), the treatment performance was 15 m3 d-1 of treatment amount in 4.5 m3 of the reactor with the average removal efficiency of 89.3% for total nitrogen and 94.9% for total phosphorus at the optimal operation condition in summer. Moreover, the average removal efficiency was 84.0% for total nitrogen and 88.3% for total phosphorus in winter although biological treatment efficiency in winter is generally lower due to bacterial lower activity. These results were revealed by the DNA barcoding analysis of 16s rRNA amplicon sequencing of samples from the sludge in winter. The comparative analysis of the bacterial community composition in sludge at the high efficiency of the system showed the predominant genera Psychromonas (significantly increased to 45.6% relative abundance), Vibrio (13.3%), Gaetbulibacter (5.7%), and Psychroserpens (4.3%) in the 4 week adaptation after adding marine sediment, suggesting that those predominant bacteria influenced the treatment performance in winter.

| S-EPMC7236998 | biostudies-literature

An Assessment of Mass Flows, Removal and Environmental Emissions of Bisphenols in a Sequencing Batch Reactor Wastewater Treatment Plant.

Project description:This study analyzed 16 bisphenols (BPs) in wastewater and sludge samples collected from different stages at a municipal wastewater treatment plant based on sequencing batch reactor technology. It also describes developing an analytical method for determining BPs in the solid phase of activated sludge based on solid-phase extraction and gas chromatography-mass spectrometry. Obtained concentrations are converted into mass flows, and the biodegradation of BPs and adsorption to primary and secondary sludge are determined. Ten of the sixteen BPs were present in the influent with concentrations up to 434 ng L-1 (BPS). Only five BPs with concentrations up to 79 ng L-1 (BPA) were determined in the plant effluent, accounting for 8 % of the total BPs determined in the influent. Eleven per cent of the total BPs were adsorbed on primary and secondary sludge. Overall, BPs biodegradation efficiency was 81%. The highest daily emissions via effluent release (1.48 g day-1) and sludge disposal (4.63 g day-1) were for BPA, while total emissions reached 2 g day-1 via effluent and 6 g day-1 via sludge disposal. The data show that the concentrations of BPs in sludge are not negligible, and their environmental emissions should be monitored and further studied.

| S-EPMC9736069 | biostudies-literature

Treatment of High-Concentration Wastewater from an Oil and Gas Field via a Paired Sequencing Batch and Ceramic Membrane Reactor.

Project description:A sequencing batch reactor (SBR) and a ceramic membrane bioreactor (CMBR) were used in conjunction (SBR+CMBR) to treat high-concentration oil and gas field wastewater (HCOGW) from the China National Offshore Oil Corporation Zhanjiang Branch (Zhanjiang, Guangdong, China). The chemical oxygen demand (COD) and the oil concentrations in the wastewater were 20,000-76,000 and 600-2200 mg/L, respectively. After the SBR+CMBR process, the effluent COD and oil content values were less than 250 mg/L and 2 mg/L, respectively, which met the third level of the Integrated Wastewater Discharge Standards of China (GB8978-1996). Through microbiological analysis, it was found that the CMBR domesticated a previously unreported functional microorganism (JF922467.1) that successfully formed a new microbial ecosystem suitable for HCOGW treatment. In conjunction with the SBR process, the CMBR process effectively reduced pollutant concentrations in HCOGW. Moreover, economic analyses indicated that the total investment required to implement the proposed infrastructure would be approximately 671,776.61 USD, and the per-unit water treatment cost would be 1.04 USD/m3.

| S-EPMC7143815 | biostudies-literature

The microbiome of two strategies for ammonia removal with the sequencing batch moving bed biofilm reactor treating cheese production wastewater.

Project description:ImportanceCheese production facilities must abide by sewage discharge bylaws that prevent overloading municipal water resource recovery facilities, eutrophication, and toxicity to aquatic life. Compact treatment systems can permit on-site treatment of cheese production wastewater; however, competition between heterotrophs and nitrifiers impedes the implementation of the sequencing batch moving bed biofilm reactor (SB-MBBR) for nitrification from high-carbon wastewaters. This study demonstrates that a single SB-MBBR is not feasible for nitrification when operated with anerobic and aerobic cycling for carbon and phosphorous removal from cheese production wastewater, as nitrification does not occur in a single reactor. Thus, two reactors in series are recommended to achieve nitrification from cheese production wastewater in SB-MBBRs. These findings can be applied to pilot and full-scale SB-MBBR operations. By demonstrating the potential to implement partial nitrification in the SB-MBBR system, this study presents the possibility of implementing partial nitrification in the SB-MBBR, resulting in the potential for more sustainable treatment of nitrogen from cheese production wastewater.

| S-EPMC10734506 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data