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Metalloriboswitches: RNA-based inorganic ion sensors that regulate genes.

ABSTRACT: Divalent ions fulfill essential cellular roles and are required for virulence by certain bacteria. Free intracellular Mg2+ can approach 5 mm, but at this level Mn2+, Ni2+, or Co2+ can be growth-inhibitory, and magnesium fluoride is toxic. To maintain ion homeostasis, many bacteria have evolved ion sensors embedded in the 5'-leader sequences of mRNAs encoding ion uptake or efflux channels. Here, we review current insights into these "metalloriboswitches," emphasizing ion-specific binding by structured RNA aptamers and associated conformational changes in downstream signal sequences. This riboswitch-effector interplay produces a layer of gene regulatory feedback that has elicited interest as an antibacterial target.

SUBMITTER: Wedekind JE

PROVIDER: S-EPMC5465474 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Metalloriboswitches: RNA-based inorganic ion sensors that regulate genes.

Wedekind Joseph E JE Dutta Debapratim D Belashov Ivan A IA Jenkins Jermaine L JL

The Journal of biological chemistry 20170428 23

Divalent ions fulfill essential cellular roles and are required for virulence by certain bacteria. Free intracellular Mg2+ can approach 5 mm, but at this level Mn2+, Ni2+, or Co2+ can be growth-inhibitory, and magnesium fluoride is toxic. To maintain ion homeostasis, many bacteria have evolved ion sensors embedded in the 5'-leader sequences of mRNAs encoding ion uptake or efflux channels. Here, we review current insights into these "metalloriboswitches ...[more]

PMID: 28455443

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