Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification.
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ABSTRACT:
SUBMITTER: Ro HS
PROVIDER: S-EPMC546681 | biostudies-literature | 2005 Feb
REPOSITORIES: biostudies-literature
Publications
Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification.
Ro Hyeon-Su HS Jung Sun Ok SO Kho Byung Hoon BH Hong Hyung Pyo HP Lee Jae Sung JS Shin Yong-Beom YB Kim Min Gon MG Chung Bong Hyun BH
Applied and environmental microbiology 20050201 2
A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins. ...[more]
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