Ontology highlight
ABSTRACT:
SUBMITTER: Rees HA
PROVIDER: S-EPMC5467206 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Nature communications 20170606
We recently developed base editing, a genome-editing approach that enables the programmable conversion of one base pair into another without double-stranded DNA cleavage, excess stochastic insertions and deletions, or dependence on homology-directed repair. The application of base editing is limited by off-target activity and reliance on intracellular DNA delivery. Here we describe two advances that address these limitations. First, we greatly reduce off-target base editing by installing mutatio ...[more]