Ontology highlight
ABSTRACT:
SUBMITTER: Kuhlmann JW
PROVIDER: S-EPMC5474721 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Kuhlmann Jan W JW Junius Meike M Diederichsen Ulf U Steinem Claudia C
Biophysical journal 20170601 11
In vitro single-vesicle fusion assays are important tools to analyze the details of SNARE-mediated fusion processes. In this study, we employed planar pore-spanning membranes (PSMs) prepared on porous silicon substrates with large pore diameters of 5 μm, allowing us to compare the process of vesicle docking and fusion on the supported parts of the PSMs (s-PSMs) with that on the freestanding membrane parts (f-PSM) under the exact same experimental conditions. The PSMs harbor the t-SNARE ΔN49-comp ...[more]