Ontology highlight
ABSTRACT:
SUBMITTER: Gessner C
PROVIDER: S-EPMC5476592 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Gessner Chris C Steinchen Wieland W Bédard Sabrina S J Skinner John J Woods Virgil L VL Walsh Thomas J TJ Bange Gert G Pantazatos Dionysios P DP
Scientific reports 20170619 1
Hydrogen-deuterium exchange (HDX) coupled with mass spectrometry (HDXMS) is a rapid and effective method for localizing and determining protein stability and dynamics. Localization is routinely limited to a peptide resolution of 5 to 20 amino acid residues. HDXMS data can contain information beyond that needed for defining protein stability at single amide resolution. Here we present a method for extracting this information from an HDX dataset to generate a HDXMS protein stability fingerprint. H ...[more]