Dataset Information

Mapping the Glyco-Gold Nanoparticles of Different Shapes Toxicity, Biodistribution and Sequestration in Adult Zebrafish.

ABSTRACT: Glyconanotechnology offers a broad range of applications across basic and translation research. Despite the tremendous progress in glyco-nanomaterials, there is still a huge gap between the basic research and therapeutic applications of these molecules. It has been reported that complexity and the synthetic challenges in glycans synthesis, the cost of the high order in vivo models and large amount of sample consumptions limited the effort to translate the glyco-nanomaterials into clinical applications. In this regards, several promising simple animal models for preliminary, quick analysis of the nanomaterials activities has been proposed. Herein, we have studied a systematic evaluation of the toxicity, biodistribution of fluorescently tagged PEG and mannose-capped gold nanoparticles (AuNPs) of three different shapes (sphere, rod, and star) in the adult zebrafish model, which could accelerate and provide preliminary results for further experiments in the higher order animal system. ICP-MS analysis and confocal images of various zebrafish organs revealed that rod-AuNPs exhibited the fast uptake. While, star-AuNPs displayed prolong sequestration, demonstrating its potential therapeutic efficacy in drug delivery.

SUBMITTER: Sangabathuni S

PROVIDER: S-EPMC5484690 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Mapping the Glyco-Gold Nanoparticles of Different Shapes Toxicity, Biodistribution and Sequestration in Adult Zebrafish.

Sangabathuni Sivakoti S Murthy Raghavendra Vasudeva RV Chaudhary Preeti Madhukar PM Subramani Balamurugan B Toraskar Suraj S Kikkeri Raghavendra R

Scientific reports 20170626 1

Glyconanotechnology offers a broad range of applications across basic and translation research. Despite the tremendous progress in glyco-nanomaterials, there is still a huge gap between the basic research and therapeutic applications of these molecules. It has been reported that complexity and the synthetic challenges in glycans synthesis, the cost of the high order in vivo models and large amount of sample consumptions limited the effort to translate the glyco-nanomaterials into clinical applic ...[more]

PMID: 28652584

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Project description:BackgroundNanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.Methodology/principal findingsPRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.Conclusions/significanceHerein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

Dataset Information

Mapping the Glyco-Gold Nanoparticles of Different Shapes Toxicity, Biodistribution and Sequestration in Adult Zebrafish.

Publications

Mapping the Glyco-Gold Nanoparticles of Different Shapes Toxicity, Biodistribution and Sequestration in Adult Zebrafish.

Similar Datasets

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