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Super-resolution structure of DNA significantly differs in buccal cells of controls and Alzheimer's patients.

ABSTRACT: The advent of super-resolution microscopy allowed for new insights into cellular and physiological processes of normal and diseased cells. In this study, we report for the first time on the super-resolved DNA structure of buccal cells from patients with Alzheimer's disease (AD) versus age- and gender-matched healthy, non-caregiver controls. In this super-resolution study cohort of 74 participants, buccal cells were collected and their spatial DNA organization in the nucleus examined by 3D Structured Illumination Microscopy (3D-SIM). Quantitation of the super-resolution DNA structure revealed that the nuclear super-resolution DNA structure of individuals with AD significantly differs from that of their controls (p?

SUBMITTER: Garcia A

PROVIDER: S-EPMC5485033 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Super-resolution structure of DNA significantly differs in buccal cells of controls and Alzheimer's patients.

Garcia Angeles A Huang David D Righolt Amanda A Righolt Christiaan C Kalaw Maria Carmela MC Mathur Shubha S McAvoy Elizabeth E Anderson James J Luedke Angela A Itorralba Justine J Mai Sabine S

Journal of cellular physiology 20170328 9

The advent of super-resolution microscopy allowed for new insights into cellular and physiological processes of normal and diseased cells. In this study, we report for the first time on the super-resolved DNA structure of buccal cells from patients with Alzheimer's disease (AD) versus age- and gender-matched healthy, non-caregiver controls. In this super-resolution study cohort of 74 participants, buccal cells were collected and their spatial DNA organization in the nucleus examined by 3D Struct ...[more]

PMID: 27996096

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