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Universal Super-Resolution Multiplexing by DNA Exchange.

ABSTRACT: Super-resolution microscopy allows optical imaging below the classical diffraction limit of light with currently up to 20× higher spatial resolution. However, the detection of multiple targets (multiplexing) is still hard to implement and time-consuming to conduct. Here, we report a straightforward sequential multiplexing approach based on the fast exchange of DNA probes which enables efficient and rapid multiplexed target detection with common super-resolution techniques such as (d)STORM, STED, and SIM. We assay our approach using DNA origami nanostructures to quantitatively assess labeling, imaging, and washing efficiency. We furthermore demonstrate the applicability of our approach by imaging multiple protein targets in fixed cells.

SUBMITTER: Schueder F

PROVIDER: S-EPMC5540260 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Universal Super-Resolution Multiplexing by DNA Exchange.

Schueder Florian F Strauss Maximilian T MT Hoerl David D Schnitzbauer Joerg J Schlichthaerle Thomas T Strauss Sebastian S Yin Peng P Harz Hartmann H Leonhardt Heinrich H Jungmann Ralf R

Angewandte Chemie (International ed. in English) 20170303 14

Super-resolution microscopy allows optical imaging below the classical diffraction limit of light with currently up to 20× higher spatial resolution. However, the detection of multiple targets (multiplexing) is still hard to implement and time-consuming to conduct. Here, we report a straightforward sequential multiplexing approach based on the fast exchange of DNA probes which enables efficient and rapid multiplexed target detection with common super-resolution techniques such as (d)STORM, STED, ...[more]

PMID: 28256790

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