Ontology highlight
ABSTRACT:
SUBMITTER: Ietswaart R
PROVIDER: S-EPMC5493185 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Ietswaart Robert R Rosa Stefanie S Wu Zhe Z Dean Caroline C Howard Martin M
Cell systems 20170614 6
Single-cell quantification of transcription kinetics and variability promotes a mechanistic understanding of gene regulation. Here, using single-molecule RNA fluorescence in situ hybridization and mathematical modeling, we dissect cellular RNA dynamics for Arabidopsis FLOWERING LOCUS C (FLC). FLC expression quantitatively determines flowering time and is regulated by antisense (COOLAIR) transcription. In cells without observable COOLAIR expression, we quantify FLC transcription initiation, elong ...[more]