Origin and evolution of group XI secretory phospholipase A₂ from flax (Linum usitatissimum) based on phylogenetic analysis of conserved domains.

ABSTRACT: Phospholipase A₂ (PLA₂) belongs to class of lipolytic enzymes (EC 3.1.1.4). Lysophosphatidic acid (LPA) and free fatty acids (FFAs) are the products of PLA₂ catalyzed hydrolysis of phosphoglycerides at sn-2 position. LPA and FFA that act as second mediators involved in the development and maturation of plants and animals. Mining of flax genome identified two phospholipase A₂ encoding genes, viz., LusPLA ₂ I and LusPLA ₂ II (Linum usitatissimum secretory phospholipase A₂). Molecular simulation of LusPLA₂s with already characterized plant sPLA₂s revealed the presence of conserved motifs and signature domains necessary to classify them as secretory phospholipase A₂. Phylogenetic analysis of flax sPLA₂ with representative sPLA₂s from other organisms revealed that they evolved rapidly via gene duplication/deletion events and shares a common ancestor. Our study is the first report of detailed phylogenetic analysis for secretory phospholipase A₂ in flax. Comparative genomic analysis of two LusPLA₂s with earlier reported plant sPLA₂s, based on their gene architectures, sequence similarities, and domain structures are presented elucidating the uniqueness of flax sPLA₂.

SUBMITTER: Gupta P 

PROVIDER: S-EPMC5494027 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature