Ontology highlight
ABSTRACT:
SUBMITTER: Chirgadze DY
PROVIDER: S-EPMC5495172 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Chirgadze Dimitri Y DY Ascher David B DB Blundell Tom L TL Sibanda Bancinyane L BL
Methods in enzymology 20170503
DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is central to the regulation of the DNA damage response and repair through nonhomologous end joining. The structure has proved challenging due to its large size and multiple HEAT repeats. We have recently reported crystals of selenomethionine-labeled DNA-PKcs complexed with native KU80ct<sub>194</sub> (KU80 residues 539-732) diffracting to 4.3Å resolution. The novel use of crystals of selenomethionine-labeled protein expressed in HeLa cel ...[more]