Ontology highlight
ABSTRACT:
SUBMITTER: Falck D
PROVIDER: S-EPMC5509673 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Falck David D Haberger Markus M Plomp Rosina R Hook Michaela M Bulau Patrick P Wuhrer Manfred M Reusch Dietmar D
Scientific reports 20170713 1
Erythropoietin (EPO) is a heavily glycosylated hormone whose recombinant forms are used for treatment of anaemia. EPO glycosylation is important for its pharmacological properties. An analytical workflow, which can determine EPO glycosylation in an accurate and high-throughput fashion from cell culture supernatant (CCS) in approximately 24 h, offers the possibility to follow changes during production. To address this challenge, we present a complete workflow consisting of protein purification, g ...[more]