Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits.
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ABSTRACT:
SUBMITTER: You S
PROVIDER: S-EPMC5510773 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits.
You Seungyong S Froberg James J Yu Junru J Haldar Manas M Sedigh Abbas A Mallik Sanku S Srivastava D K DK Choi Yongki Y
Chemical communications (Cambridge, England) 20170301 23
Using single-molecule approaches, we directly observed the dynamic interaction between HDAC8 and various ligands as well as conformational interconversions during the catalytic reaction. Statistical analysis identified key kinetic parameters, demonstrating that the enzymatic activity is highly sensitive to both minor variations in the ligand structures and small synthetic molecules. ...[more]
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