Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC3896158 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Chen Jin J Dalal Ravindra V RV Petrov Alexey N AN Tsai Albert A O'Leary Seán E SE Chapin Karen K Cheng Janice J Ewan Mark M Hsiung Pei-Lin PL Lundquist Paul P Turner Stephen W SW Hsu David R DR Puglisi Joseph D JD
Proceedings of the National Academy of Sciences of the United States of America 20131230 2
Zero-mode waveguides provide a powerful technology for studying single-molecule real-time dynamics of biological systems at physiological ligand concentrations. We customized a commercial zero-mode waveguide-based DNA sequencer for use as a versatile instrument for single-molecule fluorescence detection and showed that the system provides long fluorophore lifetimes with good signal to noise and low spectral cross-talk. We then used a ribosomal translation assay to show real-time fluidic delivery ...[more]