Steps toward Maturation of Embryonic Stem Cell-Derived Cardiomyocytes by Defined Physical Signals.
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ABSTRACT:
SUBMITTER: Shen N
PROVIDER: S-EPMC5511039 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
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Steps toward Maturation of Embryonic Stem Cell-Derived Cardiomyocytes by Defined Physical Signals.
Shen Nian N Knopf Anne A Westendorf Claas C Kraushaar Udo U Riedl Julia J Bauer Hannah H Pöschel Simone S Layland Shannon Lee SL Holeiter Monika M Knolle Stefan S Brauchle Eva E Nsair Ali A Hinderer Svenja S Schenke-Layland Katja K
Stem cell reports 20170518 1
Cardiovascular disease remains a leading cause of mortality and morbidity worldwide. Embryonic stem cell-derived cardiomyocytes (ESC-CMs) may offer significant advances in creating in vitro cardiac tissues for disease modeling, drug testing, and elucidating developmental processes; however, the induction of ESCs to a more adult-like CM phenotype remains challenging. In this study, we developed a bioreactor system to employ pulsatile flow (1.48 mL/min), cyclic strain (5%), and extended culture ti ...[more]
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