Sequence-Directed Covalent Protein-DNA Linkages in a Single Step Using HUH-Tags.
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ABSTRACT:
SUBMITTER: Lovendahl KN
PROVIDER: S-EPMC5517037 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Publications
Sequence-Directed Covalent Protein-DNA Linkages in a Single Step Using HUH-Tags.
Lovendahl Klaus N KN Hayward Amanda N AN Gordon Wendy R WR
Journal of the American Chemical Society 20170516 20
We present a robust strategy to covalently link proteins and DNA using HUH-endonuclease domains as fusion partners (HUH-tags). We show that HUH-tags react robustly with specific sequences of unmodified single-stranded DNA, and we have identified five tags that react orthogonally with distinct DNA sequences. We demonstrate the versatility of HUH-tags as fusion partners in Cas9-mediated gene editing and the construction of doubly DNA-tethered proteins for single-molecule studies. Finally we demons ...[more]
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