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Single-Cell Mechanical Characteristics Analyzed by Multiconstriction Microfluidic Channels.

ABSTRACT: A microfluidic device composed of variable numbers of multiconstriction channels is reported in this paper to differentiate a human breast cancer cell line, MDA-MB-231, and a nontumorigenic human breast cell line, MCF-10A. Differences between their mechanical properties were assessed by comparing the effect of single or multiple relaxations on their velocity profiles which is a novel measure of their deformation ability. Videos of the cells were recorded via a microscope using a smartphone, and imported to a tracking software to gain the position information on the cells. Our results indicated that a multiconstriction channel design with five deformation (50 ?m in length, 10 ?m in width, and 8 ?m in height) separated by four relaxation (50 ?m in length, 40 ?m in width, and 30 ?m in height) regions was superior to a single deformation design in differentiating MDA-MB-231 and MCF-10A cells. Velocity profile criteria can achieve a differentiation accuracy around 95% for both MDA-MB-231 and MCF-10A cells.

SUBMITTER: Ren X

PROVIDER: S-EPMC5533582 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Single-Cell Mechanical Characteristics Analyzed by Multiconstriction Microfluidic Channels.

Ren Xiang X Ghassemi Parham P Babahosseini Hesam H Strobl Jeannine S JS Agah Masoud M

ACS sensors 20170210 2

A microfluidic device composed of variable numbers of multiconstriction channels is reported in this paper to differentiate a human breast cancer cell line, MDA-MB-231, and a nontumorigenic human breast cell line, MCF-10A. Differences between their mechanical properties were assessed by comparing the effect of single or multiple relaxations on their velocity profiles which is a novel measure of their deformation ability. Videos of the cells were recorded via a microscope using a smartphone, and ...[more]

PMID: 28723132

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Dataset Information

Single-Cell Mechanical Characteristics Analyzed by Multiconstriction Microfluidic Channels.

Publications

Single-Cell Mechanical Characteristics Analyzed by Multiconstriction Microfluidic Channels.

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