?Np63? promotes adhesion of metastatic prostate cancer cells to the bone through regulation of CD82.

ABSTRACT: ?Np63? is a critical mediator of epithelial development and stem cell function in a variety of tissues including the skin and breast, while overexpression of ?Np63? acts as an oncogene to drive tumor formation and cancer stem cell properties in squamous cell carcinoma. However, with regards to the prostate, while ?Np63? is expressed in the basal stem cells of the mature gland, during adenocarcinoma development, its expression is lost and its absence is used to clinically diagnose the malignant state. Surprisingly, here we identify a sub-population of bone metastatic prostate cancer cells in the PC3 cell line that express ?Np63?. Interestingly, we discovered that ?Np63? favors adhesion and stem-like growth of these cells in the bone microenvironment. In addition, we show that these properties require expression of the target gene CD82. Together, this work uncovers a population of bone metastatic prostate cancer cells that express ?Np63?, and provides important information about the mechanisms of bone metastatic colonization. Finally, we identify metastasis-promoting properties for the tetraspanin family member CD82.

SUBMITTER: Di Giacomo V 

PROVIDER: S-EPMC5543260 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature