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IgE Trimers Drive SPE-7 Cytokinergic Activity.

ABSTRACT: Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells' receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody, SPE-7 IgE from Sigma, was found to possess the radically anomalous property, termed "cytokinergic", of inducing basophil degranulation without the intervention of an antigen. We show here that the IgE monomer, freed of protein contaminants, is devoid of this activity, and that the source of the anomaly is a trace impurity, identified as a dissociation-resistant IgE trimer. Possible models for the formation of IgE trimers and the manner in which they cross-link cell surface receptors are suggested herein.

SUBMITTER: Bax HJ

PROVIDER: S-EPMC5557881 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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IgE Trimers Drive SPE-7 Cytokinergic Activity.

Bax Heather J HJ Bowen Holly H Beavil Rebecca L RL Chung Raymond R Ward Malcolm M Davies Anna M AM Dodev Tihomir S TS McDonnell James M JM Beavil Andrew J AJ Sutton Brian J BJ Gould Hannah J HJ

Scientific reports 20170815 1

Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells' receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody, SPE-7 IgE from Sigma, was found to possess the radically anomalous property, termed "cytokinergic", of inducing basophil degranulation without the intervention of an antigen. We show here that the IgE monomer, freed of protein contaminants, is devoid of this a ...[more]

PMID: 28811536

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