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ABSTRACT:
SUBMITTER: Kampatsikas I
PROVIDER: S-EPMC5562730 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Kampatsikas Ioannis I Bijelic Aleksandar A Pretzler Matthias M Rompel Annette A
Scientific reports 20170818 1
Tyrosinases and catechol oxidases belong to the polyphenol oxidase (PPO) enzyme family, which is mainly responsible for the browning of fruits. Three cDNAs encoding PPO pro-enzymes have been cloned from leaves of Malus domestica (apple, MdPPO). The three pro-enzymes MdPPO1-3 were heterologously expressed in E. coli yielding substantial amounts of protein and have been characterized with regard to their optimum of activity resulting from SDS, acidic and proteolytic activation. Significant differe ...[more]