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SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion.

ABSTRACT: SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN premRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion. [BMB Reports 2017; 50(8): 423-428].

SUBMITTER: Moon H

PROVIDER: S-EPMC5595172 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion.

Moon Heegyum H Cho Sunghee S Loh Tiing Jen TJ Jang Ha Na HN Liu Yongchao Y Choi Namjeong N Oh Jagyeong J Ha Jiyeon J Zhou Jianhua J Cho Sungchan S Kim Dong-Eun DE Ye Michael B MB Zheng Xuexiu X Shen Haihong H

BMB reports 20170801 8

SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN premRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splic ...[more]

PMID: 28712387

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