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Wide-field imaging combined with confocal microscopy using a miniature f/5 camera integrated within a high NA objective lens.

ABSTRACT: Wide-field (WF) imaging paired with reflectance confocal microscopy can noninvasively detect skin cancer with high accuracy. However, two separate devices are required to perform each imaging procedure. We describe a new concept that integrates the two into one device: a miniature WF color camera within the objective lens used for confocal microscopy, providing simultaneous sub-surface cellular imaging and WF surface morphologic imaging. The camera, inserted between a hyperhemisphere front lens and a back lens group of the objective, commands a field of view of 4.0 mm, with a resolution better than 30 ?m, while confocal optical sectioning is preserved at sharper than 2.5 ?m.

SUBMITTER: Dickensheets DL

PROVIDER: S-EPMC5597432 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Wide-field imaging combined with confocal microscopy using a miniature f/5 camera integrated within a high NA objective lens.

Dickensheets David L DL Kreitinger Seth S Peterson Gary G Heger Michael M Rajadhyaksha Milind M

Optics letters 20170401 7

Wide-field (WF) imaging paired with reflectance confocal microscopy can noninvasively detect skin cancer with high accuracy. However, two separate devices are required to perform each imaging procedure. We describe a new concept that integrates the two into one device: a miniature WF color camera within the objective lens used for confocal microscopy, providing simultaneous sub-surface cellular imaging and WF surface morphologic imaging. The camera, inserted between a hyperhemisphere front lens ...[more]

PMID: 28362739

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