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Development of a simple measurement method for GluR2 protein expression as an index of neuronal vulnerability.

ABSTRACT: In vitro estimating strategies for potential neurotoxicity are required to screen multiple substances. In a previous study, we showed that exposure to low-concentrations of some chemicals, such as organotin, decreased the expression of GluR2 protein, which is a subunit of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type glutamate receptors, and led to neuronal vulnerability. This result suggested that GluR2 decreases as an index of neuronal cell sensitivity and vulnerability to various toxic insults. Accordingly, we developed a versatile method that is a large scale determination of GluR2 protein expression in the presence of environmental chemicals by means of AlphaLISA technology. Various analytical conditions were optimized, and then GluR2 protein amount was measured by the method using AlphaLISA. The GluR2 amounts were strongly correlated with that of measured by western blotting, which is currently used to determine GluR2 expression. An ideal standard curve could be written with the authentic GluR2 protein from 0 ng to 100 ng. Subsequently, twenty environmental chemicals were screened and nitenpyram was identified as a chemical which lead to decrease in GluR2 protein expression. This assay may provide a tool for detecting neurotoxic chemicals according to decreases in GluR2 protein expression.

SUBMITTER: Sugiyama C

PROVIDER: S-EPMC5598506 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Development of a simple measurement method for GluR2 protein expression as an index of neuronal vulnerability.

Sugiyama Chihiro C Kotake Yaichiro Y Yamaguchi Masafumi M Umeda Kanae K Tsuyama Yumi Y Sanoh Seigo S Okuda Katsuhiro K Ohta Shigeru S

Toxicology reports 20150114

<i>In vitro</i> estimating strategies for potential neurotoxicity are required to screen multiple substances. In a previous study, we showed that exposure to low-concentrations of some chemicals, such as organotin, decreased the expression of GluR2 protein, which is a subunit of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type glutamate receptors, and led to neuronal vulnerability. This result suggested that GluR2 decreases as an index of neuronal cell sensitivity and vulner ...[more]

PMID: 28962381

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Project description:Acceptance intuitively is a precondition for the adaptation and use of technology. In this systematic review, we examine academic literature on the "simple scale for acceptance measurement" provided by Van der Laan, Heino, and de Waard (1997). This measure is increasingly applied in research on mobility systems without having been thoroughly analysed. This article aims to provide such a critical analysis. We identified 437 unique references in three aggregated databases and included 128 articles (N = 6,058 participants) that empirically applied the scale in this review. The typical study focused on a mobility system using a within-subjects design in a driving simulator in Europe. Based on quality indicators of transparent study aim, group allocation procedure, variable definitions, sample characteristics, (statistical) control of confounders, reproducibility, and reporting of incomplete data and test performance, many of the 128 articles exhibited room for improvements (44% below.50; range 0 to 1). Twenty-eight studies (22%) reported reliability coefficients providing evidence that the scale and its sub-scales produce reliable results (median Cronbach's α >.83). Missing data from the majority of studies limits this conclusion. Only 2 out of 10 factor analyses replicated the proposed two-dimensional structure questioning the use of these sub-scales. Correlation results provide evidence for convergent validity of acceptance, usefulness, and satisfying with limited confidence, since only 14 studies with a median sample size of N = 40 reported correlation coefficients. With these results, the scale might be a valuable addition for technology attitude research. Firstly, we recommend thorough testing for a better understanding of acceptance, usefulness, and satisfying. Secondly, we suggest to report scale results more transparently and rigorously to enable meta-analyses in the future. The study protocol is available at the Open Science Framework (https://osf.io/j782c/).

Dataset Information

Development of a simple measurement method for GluR2 protein expression as an index of neuronal vulnerability.

Publications

Development of a simple measurement method for GluR2 protein expression as an index of neuronal vulnerability.

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