Ontology highlight
ABSTRACT:
SUBMITTER: Chow RD
PROVIDER: S-EPMC5614841 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Chow Ryan D RD Guzman Christopher D CD Wang Guangchuan G Schmidt Florian F Youngblood Mark W MW Ye Lupeng L Errami Youssef Y Dong Matthew B MB Martinez Michael A MA Zhang Sensen S Renauer Paul P Bilguvar Kaya K Gunel Murat M Sharp Phillip A PA Zhang Feng F Platt Randall J RJ Chen Sidi S
Nature neuroscience 20170814 10
A causative understanding of genetic factors that regulate glioblastoma pathogenesis is of central importance. Here we developed an adeno-associated virus-mediated, autochthonous genetic CRISPR screen in glioblastoma. Stereotaxic delivery of a virus library targeting genes commonly mutated in human cancers into the brains of conditional-Cas9 mice resulted in tumors that recapitulate human glioblastoma. Capture sequencing revealed diverse mutational profiles across tumors. The mutation frequencie ...[more]