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A flow cytometry assay to quantify intercellular exchange of membrane components.

ABSTRACT: Membrane-compound exchange is vital for cell-to-cell communication, yet quantification of this process is difficult. Here we present a method using flow cytometry in combination with bioorthogonal and fluorescent labelling techniques to quantify the amount of exchange of cholesterol and sialylated compounds between cells. We demonstrate that direct cell-cell contact is the likely mechanism of sterol-exchange and show that by manipulating the contact time between cells using complementary coiled-coil peptides results in an enhanced exchange rate of membrane components between cells.

SUBMITTER: Poulcharidis D

PROVIDER: S-EPMC5618768 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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A flow cytometry assay to quantify intercellular exchange of membrane components.

Poulcharidis Dimitrios D Belfor Kimberley K Kros Alexander A van Kasteren Sander I SI

Chemical science 20170524 8

Membrane-compound exchange is vital for cell-to-cell communication, yet quantification of this process is difficult. Here we present a method using flow cytometry in combination with bioorthogonal and fluorescent labelling techniques to quantify the amount of exchange of cholesterol and sialylated compounds between cells. We demonstrate that direct cell-cell contact is the likely mechanism of sterol-exchange and show that by manipulating the contact time between cells using complementary coiled- ...[more]

PMID: 28970937

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A flow cytometry assay to quantify intercellular exchange of membrane components.

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A flow cytometry assay to quantify intercellular exchange of membrane components.

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