Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.
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ABSTRACT:
SUBMITTER: Wong P
PROVIDER: S-EPMC7806516 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Publications
Flow cytometry-based <i>ex vivo</i> murine NK cell cytotoxicity assay.
Wong Pamela P Wagner Julia A JA Berrien-Elliott Melissa M MM Schappe Timothy T Fehniger Todd A TA
STAR protocols 20210112 1
Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure <i>in vivo</i> activated murine NK cells' ability to kill target cells <i>ex vivo</i>. Existing published protocols for assaying <i>ex vivo</i> NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol details specifically an <i>ex vivo</i> cytotoxicity assay using primary murine NK cells enriched from splenocytes that ...[more]
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