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Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana.

ABSTRACT: Zearalenone (ZEN) is a mycotoxin which causes huge economic losses in the food and animal feed industries. The lactonase ZHD101 from Clonostachys rosea, which catalyzes the hydrolytic degradation of ZEN, is the only known ZEN-detoxifying enzyme. Here, a protein homologous to ZHD101, denoted CbZHD, from Cladophialophora batiana was expressed and characterized. Sequence alignment indicates that CbZHD possesses the same catalytic triad and ZEN-interacting residues as found in ZHD101. CbZHD exhibits optimal enzyme activity at 35°C and pH 8, and is sensitive to heat treatment. The crystal structure of apo CbZHD was determined to 1.75?Å resolution. The active-site compositions of CbZHD and ZHD101 were analyzed.

SUBMITTER: Hui R

PROVIDER: S-EPMC5619743 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana.

Hui Renjie R Hu Xiangying X Liu Wenting W Liu Weidong W Zheng Yingying Y Chen Yun Y Guo Rey Ting RT Jin Jian J Chen Chun Chi CC

Acta crystallographica. Section F, Structural biology communications 20170821 Pt 9

Zearalenone (ZEN) is a mycotoxin which causes huge economic losses in the food and animal feed industries. The lactonase ZHD101 from Clonostachys rosea, which catalyzes the hydrolytic degradation of ZEN, is the only known ZEN-detoxifying enzyme. Here, a protein homologous to ZHD101, denoted CbZHD, from Cladophialophora batiana was expressed and characterized. Sequence alignment indicates that CbZHD possesses the same catalytic triad and ZEN-interacting residues as found in ZHD101. CbZHD exhibits ...[more]

PMID: 28876230

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