Filopodyan: An open-source pipeline for the analysis of filopodia.
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ABSTRACT:
SUBMITTER: Urbancic V
PROVIDER: S-EPMC5626553 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Publications
Filopodyan: An open-source pipeline for the analysis of filopodia.
Urbančič Vasja V Butler Richard R Richier Benjamin B Peter Manuel M Mason Julia J Livesey Frederick J FJ Holt Christine E CE Gallop Jennifer L JL
The Journal of cell biology 20170731 10
Filopodia have important sensory and mechanical roles in motile cells. The recruitment of actin regulators, such as ENA/VASP proteins, to sites of protrusion underlies diverse molecular mechanisms of filopodia formation and extension. We developed Filopodyan (filopodia dynamics analysis) in Fiji and R to measure fluorescence in filopodia and at their tips and bases concurrently with their morphological and dynamic properties. Filopodyan supports high-throughput phenotype characterization as well ...[more]
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