Ontology highlight
ABSTRACT:
SUBMITTER: Menon BRK
PROVIDER: S-EPMC5637929 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Menon Binuraj R K BRK Brandenburger Eileen E Sharif Humera H HH Klemstein Ulrike U Shepherd Sarah A SA Greaney Michael F MF Micklefield Jason J
Angewandte Chemie (International ed. in English) 20170818 39
Flavin-dependent halogenases are useful enzymes for providing halogenated molecules with improved biological activity, or intermediates for synthetic derivatization. We demonstrate how the fungal halogenase RadH can be used to regioselectively halogenate a range of bioactive aromatic scaffolds. Site-directed mutagenesis of RadH was used to identify catalytic residues and provide insight into the mechanism of fungal halogenases. A high-throughput fluorescence screen was also developed, which enab ...[more]