Translesion synthesis DNA polymerase ? exhibits a specific RNA extension activity and a transcription-associated function.

ABSTRACT: Polymerase eta (Pol?) is a low fidelity translesion synthesis DNA polymerase that rescues damage-stalled replication by inserting deoxy-ribonucleotides opposite DNA damage sites resulting in error-free or mutagenic damage bypass. In this study we identify a new specific RNA extension activity of Pol? of Saccharomyces cerevisiae. We show that Pol? is able to extend RNA primers in the presence of ribonucleotides (rNTPs), and that these reactions are an order of magnitude more efficient than the misinsertion of rNTPs into DNA. Moreover, during RNA extension Pol? performs error-free bypass of the 8-oxoguanine and thymine dimer DNA lesions, though with a 103 and 102-fold lower efficiency, respectively, than it synthesizes opposite undamaged nucleotides. Furthermore, in vivo experiments demonstrate that the transcription of several genes is affected by the lack of Pol?, and that Pol? is enriched over actively transcribed regions. Moreover, inactivation of its polymerase activity causes similar transcription inhibition as the absence of Pol?. In summary, these results suggest that the new RNA synthetic activity of Pol? can have in vivo relevance.

SUBMITTER: Gali VK 

PROVIDER: S-EPMC5638924 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature