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A genetically encoded cyclobutene probe for labelling of live cells.

ABSTRACT: We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels-Alder reaction with a tetrazine.

SUBMITTER: Liu K 

PROVIDER: S-EPMC5648060 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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A genetically encoded cyclobutene probe for labelling of live cells.

Liu K K   Enns B B   Evans B B   Wang N N   Shang X X   Sittiwong W W   Dussault P H PH   Guo J J  

Chemical communications (Cambridge, England) 20170901 76

We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels-Alder reaction with a tetrazine. ...[more]

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