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Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behcet's disease.

ABSTRACT: Studies have suggested that soluble factors in plasma from patients with active (aBD) and inactive (iBD) Behçet's disease (BD) stimulate neutrophil function. Soluble CD40 ligand (sCD40L) is an important mediator of inflammation in BD. Its expression and effect on neutrophil oxidative burst and neutrophil extracellular trap (NET) release have not been characterized. In this study, we sought to investigate the role of plasma and the CD40L pathway on NET release and the oxidative burst profile in patients with aBD and iBD.Neutrophils and peripheral blood mononuclear cells (PBMCs) were obtained from patients with aBD (n?=?30), patients with iBD (n?=?31), and healthy control subjects (HCs; n?=?30). sCD40L plasma concentration was determined in individual samples. A pool of plasma for each group was created. In some experiments, plasma pools were treated with recombinant CD40 (rhCD40-muIg) for sCD40L blockade. NET release and H2O2/O2- production were determined after stimulation with phorbol 12-myristate 13-acetate, sCD40L, or plasma pool. Flow cytometric analysis was performed to evaluate the expression of (1) CD40, Mac-1, and phosphorylated NF-?B p65 on neutrophils and monocytes and (2) CD40L on activated T cells and platelets. CD40L gene expression in PBMCs was determined by qRT-PCR.sCD40L plasma levels were significantly higher in patients with iBD (median 17,234, range 2346-19,279 pg/ml) and patients with aBD (median 18,289, range 413-19,883 pg/ml) than in HCs (median 47.5, range 33.7-26.7 pg/ml; p?

SUBMITTER: Perazzio SF

PROVIDER: S-EPMC5649058 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet's disease.

Perazzio Sandro Félix SF Soeiro-Pereira Paulo Vitor PV Dos Santos Viviane Cardoso VC de Brito Marlon Vilela MV Salu Bruno B Oliva Maria Luiza Vilela MLV Stevens Anne Margherite AM de Souza Alexandre Wagner Silva AWS Ochs Hans D HD Torgerson Troy R TR Condino-Neto Antonio A Andrade Luis Eduardo Coelho LEC

Arthritis research & therapy 20171019 1

<h4>Background</h4>Studies have suggested that soluble factors in plasma from patients with active (aBD) and inactive (iBD) Behçet's disease (BD) stimulate neutrophil function. Soluble CD40 ligand (sCD40L) is an important mediator of inflammation in BD. Its expression and effect on neutrophil oxidative burst and neutrophil extracellular trap (NET) release have not been characterized. In this study, we sought to investigate the role of plasma and the CD40L pathway on NET release and the oxidative ...[more]

PMID: 29052524

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Dataset Information

Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behcet's disease.

Publications

Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet's disease.

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