Dataset Information

Evidence of disordered calcium metabolism in adolescent girls with type 1 diabetes: An observational study using a dual-stable calcium isotope technique.

ABSTRACT: Type 1 diabetes (T1D) is associated with skeletal abnormalities including low bone density and increased fracture risk. The pathophysiology underlying T1D related skeletal fragility remains unknown. The objective of this study was to use a dual-stable calcium isotope method to investigate the effects of T1D on calcium absorption and estimated calcium retention in adolescent females. Twenty adolescent females with T1D were admitted for a 24-h calcium absorption study using oral (44Ca) and intravenous (42Ca) stable isotopes for determination of percent gastrointestinal calcium absorption and estimated calcium retention. Five out of twenty participants were found to have negative estimated calcium retention. Participants with negative calcium retention had greater urinary calcium excretion [202mg/d (IQR: 178-213)] compared to those with positive calcium retention [101.5mg/d (IQR: 82-122)], p=0.01, but similar calcium intake and percent calcium absorption. With the exception of one outlier, 24-h urine calcium was significantly associated with hemoglobin A1c (Pearson's r=0.55, p=0.02). 50% of participants consumed less than the RDA for calcium; fractional calcium absorption was inversely correlated with calcium intake in participants not meeting the RDA (Spearman's rho -0.65, p=0.04). In conclusion, one-quarter of adolescent girls with T1D were found to have negative estimated calcium retention at a time when bone mineral accrual should be ongoing. This appeared to be the result of excess urinary calcium excretion as opposed to diminished gastrointestinal calcium absorption. Insufficient calcium availability for bone deposition during adolescence could impair bone mineral accrual and contribute to skeletal fragility. Trial registered: ClinicalTrials.gov Reg No. NCT03156179.

SUBMITTER: Weber DR

PROVIDER: S-EPMC5650924 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Evidence of disordered calcium metabolism in adolescent girls with type 1 diabetes: An observational study using a dual-stable calcium isotope technique.

Weber David R DR O'Brien Kimberly O KO Schwartz George J GJ

Bone 20170904

Type 1 diabetes (T1D) is associated with skeletal abnormalities including low bone density and increased fracture risk. The pathophysiology underlying T1D related skeletal fragility remains unknown. The objective of this study was to use a dual-stable calcium isotope method to investigate the effects of T1D on calcium absorption and estimated calcium retention in adolescent females. Twenty adolescent females with T1D were admitted for a 24-h calcium absorption study using oral (<sup>44</sup>Ca) ...[more]

PMID: 28882565

Similar Datasets

Project description:To investigate the underlying mechanisms of low metabolic activity of primary chondrocytes obtained from girls with adolescent idiopathic scoliosis (AIS); AIS is a spine-deforming disease that often occurs in girls. AIS is associated with a lower bone mass than that of healthy individuals and osteopenia. Leptin was shown to play an important role in bone growth. It can also regulate the function of chondrocytes. Changes in leptin and Ob-R levels in AIS patients have been reported in several studies. The underlying mechanisms between the dysfunction of peripheral leptin signaling and abnormal chondrocytes remain unclear; The following parameters were evaluated in AIS patients and the control groups: total serum leptin levels; Ob-R expression in the plasma membrane of primary chondrocytes; JAK2 and STAT3 phosphorylation status. Then, we inhibited the lysosome and proteasome and knocked down clathrin heavy chain (CHC) expression in primary chondrocytes isolated from girls with AIS and evaluated Ob-R expression. We investigated the effects of leptin combined with a lysosome inhibitor or CHC knockdown in primary chondrocytes obtained from AIS patients; Compared with the controls, AIS patients showed similar total serum leptin levels, reduced JAK2 and STAT3 phosphorylation, and decreased cartilage matrix synthesis in the facet joint. Lower metabolic activity and lower membrane expression of Ob-R were observed in primary chondrocytes from the AIS group than in the controls. Lysosome inhibition increased the total Ob-R content but had no effect on the membrane expression of Ob-R or leptin's effects on AIS primary chondrocytes. CHC knockdown upregulated the membrane Ob-R levels and enhanced leptin's effects on AIS primary chondrocytes; The underlying mechanism of chondrocytes that are hyposensitive to leptin in some girls with AIS is low plasma membrane Ob-R expression that results from an imbalance between the rate of receptor endocytosis and the insertion of newly synthesized receptors into the membrane.

Project description:Background:Iron nutrition is important for the health of women of reproductive age, and defining the physiologic requirement for iron can help them accurately plan the iron intake. However, research on the physiologic requirement for iron in women is insufficient worldwide. This study aimed to further improve the methodology and get more precise data for the physiological requirements for iron in women of reproductive age on the basis of our previous study. Method:Sixty-one women of reproductive age who had not been pregnant before and during the whole study were included from Hebei province, China in 2015. Each subject participated in a 2-week metabolic trial with consuming 50?mg of the stable isotope 58Fe, and were then followed for ~?800?days. The abundance of 58Fe and the total iron concentration in the circulation were measured using multi-collector inductively-coupled plasma mass spectrometry and atomic absorption spectroscopy. The physiologic requirement for iron in women of reproductive age was then calculated. Results:The average iron circulation rate was 80.4%, and the steady period started from about 1?year. The average physiological requirement for iron of 21 subjects obtained by formula calculation was 1.55?mg/d and 23.63??g.kg-?1.d-?1 after adjustment for body mass, and that of 33 subjects obtained by linear regression was 1.29?mg/d, 20.98??g.kg-?1.d-?1 after adjustment for body mass. The results by two methods showed no significant difference. The EAR and RNI calculated from this results was 11-13?mg/d and 15-18?mg/d, respectively, both of which were slightly lower than the recommended value in Chinese Dietary Reference Intake (2013). Conclusion:The physiological requirements for iron in women of reproductive age were in accordance with other studies, while the EAR and RNI calculated from which were slightly lower than Chinese present recommended value. Trial registration:ChiCTR, ChiCTR-OCH-14004302. Registered 14 February 2014, http://www.chictr.org.cn/enindex.aspx.

Dataset Information

Evidence of disordered calcium metabolism in adolescent girls with type 1 diabetes: An observational study using a dual-stable calcium isotope technique.

Publications

Evidence of disordered calcium metabolism in adolescent girls with type 1 diabetes: An observational study using a dual-stable calcium isotope technique.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets