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Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in mice.


ABSTRACT: Marburg virus (MARV) causes severe haemorrhagic fever in humans and nonhuman primates and has a high mortality rate. However, effective drugs or licensed vaccines are not currently available to control the outbreak and spread of this disease.In this study, we generated MARV virus-like particles (VLPs) by co-expressing the glycoprotein (GP) and matrix protein (VP40) using the baculovirus expression system. MARV VLPs and three adjuvants, Poria cocos polysaccharide (PCP-II), poly(I:C) and aluminium hydroxide, were evaluated after intramuscular vaccination in mice.Murine studies demonstrated that vaccination with the MARV VLPs induce neutralizing antibodies and cellar immune responses. MARV VLPs and the PCP-II adjuvant group resulted in high titres of MARV-specific antibodies, activated relatively higher numbers of B cells and T cells in peripheral blood mononuclear cells (PBMCs), and induced greater cytokine secretion from splenocytes than the other adjuvants.MARV VLPs with the PCP-II adjuvant may constitute an effective vaccination and PCP-II should be further investigated as a novel adjuvant.

SUBMITTER: Gai W 

PROVIDER: S-EPMC5657058 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in mice.

Gai Weiwei W   Zheng Xuexing X   Wang Chong C   Wang Hualei H   Zhao Yongkun Y   Wang Qi Q   Wong Gary G   Zhang Weijiao W   Feng Na N   Qiu Boning B   Chi Hang H   Li Nan N   Wang Tiecheng T   Gao Yuwei Y   Shan Junjie J   Yang Songtao S   Xia Xianzhu X  

Virology journal 20171025 1


<h4>Background</h4>Marburg virus (MARV) causes severe haemorrhagic fever in humans and nonhuman primates and has a high mortality rate. However, effective drugs or licensed vaccines are not currently available to control the outbreak and spread of this disease.<h4>Methods</h4>In this study, we generated MARV virus-like particles (VLPs) by co-expressing the glycoprotein (GP) and matrix protein (VP40) using the baculovirus expression system. MARV VLPs and three adjuvants, Poria cocos polysaccharid  ...[more]

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