Ontology highlight
ABSTRACT:
SUBMITTER: Dittmore A
PROVIDER: S-EPMC5661871 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Physical review letters 20171003 14
We present a method of detecting sequence defects by supercoiling DNA with magnetic tweezers. The method is sensitive to a single mismatched base pair in a DNA sequence of several thousand base pairs. We systematically compare DNA molecules with 0 to 16 adjacent mismatches at 1 M monovalent salt and 3.6 pN force and show that under these conditions, a single plectoneme forms and is stably pinned at the defect. We use these measurements to estimate the energy and degree of end-loop kinking at def ...[more]