A rational approach to enhancing antibody Fc homodimer formation for robust production of antibody mixture in a single cell line.

ABSTRACT: Combinations of different antibodies have been shown to be more effective for managing certain diseases than monotherapy. Co-expression of the antibody mixture in a single cell line is key to reducing complexity during antibody development and manufacturing. However, co-transfection of multiple light and heavy chains into cells often leads to production of mismatched, heterodimeric by-products that are inactive, making the development of co-expression systems that robustly and efficiently produce highly active antibody mixtures a high priority. In this study, we modified the CH3 domain interface of the antibody fragment crystallizable (Fc) region by changing several charge pairs to create electrostatic interactions favoring Fc homodimer formation and disfavoring Fc heterodimer formation. W

SUBMITTER: Yu J 

PROVIDER: S-EPMC5663886 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature