Unknown

Dataset Information

0

Decrease in Lymphoid Specific Helicase and 5-hydroxymethylcytosine Is Associated with Metastasis and Genome Instability.


ABSTRACT: DNA methylation is an important epigenetic modification as a hallmark in cancer. Conversion of 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) by ten-eleven translocation (TET) family enzymes plays an important biological role in embryonic stem cells, development, aging and disease. Lymphoid specific helicase (LSH), a chromatin remodeling factor, is regarded as a reader of 5-hmC. Recent reports show that the level of 5-hmC is altered in various types of cancers. However, the change in 5-hmC levels in cancer and associated metastasis is not well defined. We report that the level of 5-hmC was decreased in metastatic tissues of nasopharyngeal carcinoma, breast cancer, and colon cancer relative to that in non-metastasis tumor tissues. Furthermore, our data show that TET2, but not TET3, interacted with LSH, whereas LSH increased TET2 expression through silencing miR-26b-5p and miR-29c-5p. Finally, LSH promoted genome stability by silencing satellite expression by affecting 5-hmC levels in pericentromeric satellite repeats, and LSH was resistant to cisplatin-induced DNA damage. Our data indicate that 5-hmC might serve as a metastasis marker for cancer and that the decreased expression of LSH is likely one of the mechanisms of genome instability underlying 5-hmC loss in cancer.

SUBMITTER: Jia J 

PROVIDER: S-EPMC5667415 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

altmetric image

Publications

Decrease in Lymphoid Specific Helicase and 5-hydroxymethylcytosine Is Associated with Metastasis and Genome Instability.

Jia Jiantao J   Shi Ying Y   Chen Ling L   Lai Weiwei W   Yan Bin B   Jiang Yiqun Y   Xiao Desheng D   Xi Sichuan S   Cao Ya Y   Liu Shuang S   Cheng Yan Y   Tao Yongguang Y  

Theranostics 20170905 16


DNA methylation is an important epigenetic modification as a hallmark in cancer. Conversion of 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) by ten-eleven translocation (TET) family enzymes plays an important biological role in embryonic stem cells, development, aging and disease. Lymphoid specific helicase (LSH), a chromatin remodeling factor, is regarded as a reader of 5-hmC. Recent reports show that the level of 5-hmC is altered in various types of cancers. However, the change in  ...[more]

Similar Datasets

| S-EPMC3314950 | biostudies-literature
| S-EPMC8820600 | biostudies-literature
| S-EPMC8275684 | biostudies-literature
| S-EPMC10691353 | biostudies-literature
| S-EPMC3650038 | biostudies-literature
| S-EPMC5595132 | biostudies-literature
| S-EPMC7466159 | biostudies-literature
| S-EPMC7524730 | biostudies-literature
2024-06-28 | GSE268934 | GEO