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Yeast Sen1 helicase protects the genome from transcription-associated instability.


ABSTRACT: Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form during transcription, when nascent RNA hybridizes to DNA prior to its packaging into RNA protein complexes. These hybrids displace the nontranscribed strand and create R loop structures. Loss of Sen1 results in transient R loop accumulation and so elicits transcription-associated recombination. SEN1 genetically interacts with DNA repair genes, suggesting that R loop resolution requires proteins involved in homologous recombination. Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability.

SUBMITTER: Mischo HE 

PROVIDER: S-EPMC3314950 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Yeast Sen1 helicase protects the genome from transcription-associated instability.

Mischo Hannah E HE   Gómez-González Belén B   Grzechnik Pawel P   Rondón Ana G AG   Wei Wu W   Steinmetz Lars L   Aguilera Andrés A   Proudfoot Nick J NJ  

Molecular cell 20110101 1


Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form during transcription, when nascent RNA hybridizes to DNA prior to its packaging into RNA protein complexes. These hybrids displace the nontranscribed strand and create R loop structure  ...[more]

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