Chemical Synthesis of GPI Glycan-Peptide Conjugates by Traceless Staudinger Ligation.

ABSTRACT: A new strategy has been developed for GPI glycan-peptide conjugate synthesis based upon a traceless Staudinger reaction between a peptide phosphinothioester and a GPI glycan azide. The strategy was first studied and optimized with simple peptides and GPI glycans, which offered excellent yields of the desired conjugates in both organic and aqueous solvents. It was then used to successfully synthesize an analogue of the human CD52 antigen containing the whole CD52 peptide sequence and the conserved trimannose motif of all GPI anchors.

SUBMITTER: Zhu S 

PROVIDER: S-EPMC5668870 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature