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Identification and characterization of 5?-cyprinol-sulfating cytosolic sulfotransferases (Sults) in the zebrafish (Danio rerio).

ABSTRACT: 5?-Cyprinol 27-sulfate is the major biliary bile salt present in cypriniform fish including the zebrafish (Danio rerio). The current study was designed to identify the zebrafish cytosolic sulfotransferase (Sult) enzyme(s) capable of sulfating 5?-cyprinol and to characterize the zebrafish 5?-cyprinol-sulfating Sults in comparison with human SULT2A1. Enzymatic assays using zebrafish homogenates showed 5?-cyprinol-sulfating activity. A systematic analysis, using a panel of recombinant zebrafish Sults, revealed two Sult2 subfamily members, Sult2st2 and Sult2st3, as major 5?-cyprinol-sulfating Sults. Both enzymes showed higher activities using 5?-cyprinol as the substrate, compared to their activity with DHEA, a representative substrate for mammalian SULT2 family members, particularly SULT2A1. pH-Dependence and kinetics experiments indicated that the catalytic properties of zebrafish Sult2 family members in mediating the sulfation of 5?-cyprinol were different from those of either zebrafish Sult3st4 or human SULT2A1. Collectively, these results imply that both Sult2st2 and Sult2st3 have evolved to sulfate specifically C27-bile alcohol, 5?-cyprinol, in Cypriniform fish, whereas the enzymatic characteristics of zebrafish Sult3 members, particularly Sult3st4, correlated with those of human SULT2A1.

SUBMITTER: Kurogi K

PROVIDER: S-EPMC5675747 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Identification and characterization of 5α-cyprinol-sulfating cytosolic sulfotransferases (Sults) in the zebrafish (Danio rerio).

Kurogi Katsuhisa K Yoshihama Maki M Horton Austin A Schiefer Isaac T IT Krasowski Matthew D MD Hagey Lee R LR Williams Frederick E FE Sakakibara Yoichi Y Kenmochi Naoya N Suiko Masahito M Liu Ming-Cheh MC

The Journal of steroid biochemistry and molecular biology 20170812

5α-Cyprinol 27-sulfate is the major biliary bile salt present in cypriniform fish including the zebrafish (Danio rerio). The current study was designed to identify the zebrafish cytosolic sulfotransferase (Sult) enzyme(s) capable of sulfating 5α-cyprinol and to characterize the zebrafish 5α-cyprinol-sulfating Sults in comparison with human SULT2A1. Enzymatic assays using zebrafish homogenates showed 5α-cyprinol-sulfating activity. A systematic analysis, using a panel of recombinant zebrafish Sul ...[more]

PMID: 28807679

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Dataset Information

Identification and characterization of 5?-cyprinol-sulfating cytosolic sulfotransferases (Sults) in the zebrafish (Danio rerio).

Publications

Identification and characterization of 5α-cyprinol-sulfating cytosolic sulfotransferases (Sults) in the zebrafish (Danio rerio).

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