Ontology highlight
ABSTRACT:
SUBMITTER: Zwolak A
PROVIDER: S-EPMC5680793 | biostudies-literature | 2017 Nov/Dec
REPOSITORIES: biostudies-literature
Zwolak Adam A Armstrong Anthony A AA Tam Susan H SH Pardinas Jose R JR Goulet Dennis R DR Zheng Songmao S Brosnan Kerry K Emmell Eva E Luo Jeffrey J Gilliland Gary L GL Chiu Mark L ML
mAbs 20170912 8
The increased number of bispecific antibodies (BsAb) under therapeutic development has resulted in a need for mouse surrogate BsAbs. Here, we describe a one-step method for generating highly pure mouse BsAbs suitable for in vitro and in vivo studies. We identify two mutations in the mouse IgG2a and IgG2b Fc region: one that eliminates protein A binding and one that enhances protein A binding by 8-fold. We show that BsAbs harboring these mutations can be purified from the residual parental monocl ...[more]